Fig. 4. Cardiogenic marker expression: Analysis of cardiogenic marker expression on mRNA (A) and protein (B) levels by comparison of W4 αMHC and W4 αMHC hNkx2.5 on day 25 of differentiation. (A) Relative expression of cardiac structural and gap junction proteins as well as funny channel marker performed by qPCR. Values normalized to mHprt and mPolr2a and relativized to W4 αMHC (set: 1). Values are presented as mean ± SEM; n=3; statistic was performed as t-test, *p ≤ 0.05. (B) Immunostaining of 1: α-actinin (red) , 2: Cx43 (red, arrow) and cTnT (green), 3: Myh6 (red) and Cx43 (green, arrow) and 4: Myh7 (red) and Actin (green) with counterstaining of nuclei (blue). Scale bar: 20 μm.